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技術文章
更新(xīn)時(shí)間:2023-03-07
點擊(jī)次(cì)數(shù):1589
EZBioscience是ZScience Biotechnology Corporation Limited是(shì)美國的(dí)一個生物(wù)技術品(pǐn)牌,主(zhǔ)要專(zhuān)注於新技(jì)術(shù)開(kāi)發和製造用(yòng)於分(fēn)子生物學(xué)的智能(néng)工具(jù)。EZBioscience的科(kē)學家和工(gōng)程師(shī)是(shì)蛋白(bái)質(zhì)表(biǎo)達,純(chún)化,結(jié)晶(jīng),結構測(cè)定和化學合成方麵的專家,在(zài)分子克隆,RNA純(chún)化(huà)和相關檢(jiǎn)測領域(yù)也(yě)擁(yōng)有高(gāo)度復(fù)雜(zá)的技術體(tǐ)係。EZBioscience的(dí)使命是(shì)通過向世界(jiè)各地的科(kē)學(xué)家提(tí)供(gōng)設計更(gēng)巧(qiǎo)妙的試(shì)劑和(hé)試劑(jì)盒(hé),幫助(zhù)使生物和醫學研(yán)究(jiū)更(gēng)容(róng)易(yì)。EZBioscience對醫(yī)療研(yán)究(jiū)人員的(dí)承(chéng)諾(nuò)是(shì)以優惠的(dí)價格提供(gōng)優(yōu)質(zhì)的產(chǎn)品。EZBioscience,使研(yán)究更容(róng)易(yì)。

EZBioscience熱(rè)銷產品
產品(pǐn)介(jiè)紹:The EZBioscience® EZ-press SINGLE Cell to cDNA Kit allows researchers to analyze the gene expression of single or very low cell numbers (1 ~ 1,000 cells) in a reliable, sensitive and easy way. Cells are first lysed by the unique Cell Lysis Buffer. Then cDNA is produced and pre-amplified using gene specific primers from the cell lysate in the same tube. Due to the pre-amplification following reverse transcription, extremely low concentration of RNA from a very small amount of cells can be detected by the subsequent qPCR.

產品(pǐn)介紹:The EZBioscience® EZ-press RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from a wide variety of sources, without the need for toxic substances such as phenol or chloroform. The kit can be used with cultured cells. Biological samples are first lysed and homogenized in a strong denaturant containing buffer, which immediately inactivates RNases to ensure isolation of intact RNA. After homogenization, ethanol is added to the lysate, creating conditions that promote selective binding of RNA to the silica-based membrane. The sample is then applied to the Spin Column, where the total RNA binds to the membrane, then the membrane is treated with gDNA Remover to eliminate the DNA. Contaminants are efficiently washed away by subsequent step of washing. The high-quality total RNA is then eluted in Elution Buffer. The purified total RNA is suitable for use in a variety of downstream applications, including: RT-PCR, RT-qPCR, and so on.
產品(pǐn)介紹:TheEZBioscience®4× EZscript Reverse Transcription Mix II (with gDNA Remover) is a new-generation fast reverse transcription kit with higher reverse transcription efficiency compared to previous generations.
The kit is suitable for real-time RT-PCR (RT-qPCR) that contains a gDNA Remover which can effectively eliminate the contamination of genomic DNA (gDNA) or other double stranded DNA during the analysis of gene expression. To accurately analyze gene expression, it is necessary to detect cDNA in samples without contaminating DNA. To avoid amplification of gDNA, primers can be designed on different exons spanning introns. However, there may be cases where a suitable primer cannot be designed, as with a gene with a single exon or a gene without a long intron. Also, it may be difficult to avoid unexpected amplification from gDNA due to non-specific amplification or the existence of pseudo-genes. Moreover, some labs are heavily contaminated by the PCR products of previous tests. This kit offers a potent gDNA Remover that can eliminate double stranded DNA in RNA sample in 5 minutes at room temperature without loss of RNA. Then the first strand of cDNA is synthesized by adding the 4× EZscript RT Mix II. Reaction products are applicable to subsequent PCR, qPCR.
The mix contains reverse transcriptase, RNase Inhibitor, optimized buffer system, dNTPs, and Oligo dT18 and Random Hexamer as primers. The reverse transcriptase in this Mix is a genetic engineered enzyme based on M-MLV (RNase H-) reverse transcriptase. And the multiple site-mutations of reverse transcriptase can obviously increase its affinity to RNA templates and its strand extending ability, which make reverse transcription reaction more efficient. Moreover, this transcriptase is rather resistant to common reverse transcriptase inhibitors. At the same time, the kit uses the latest optimized reaction system to further improve the reverse transcription efficiency. This product is also very suitable for reverse transcription using plant RNA.
EZBioscience代(dài)理(lǐ)——天(tiān)津益元利(lì)康科技有限公(gōng)司(sī),提供EZBioscience全係(xì)列產(chǎn)品。EZBioscience華(huá)中代理,EZBioscience華北代理,EZBioscience華(huá)南代(dài)理,EZBioscience華東(dōng)代理。天津益元利康科技有(yǒu)限公司作(zuò)為(wéi)一家專(zhuān)注於免疫學,細(xì)胞(bāo)生物(wù)學,分子生物(wù)學(xué)等領域核心試(shì)劑產品的研發,生產(chǎn)與銷(xiāo)售的高新(xīn)企業。天津(jīn)益(yì)元(yuán)利(lì)康科技有(yǒu)限公司(sī)依托於中科院的(dí)科(kē)研技術力(lì)量(liáng),以中(zhōng)科院資(zī)深科(kē)學家為(wéi)核心,整合(hé)經(jīng)驗豐富的商(shāng)業團隊及(jí)管理(lǐ)團隊組建(jiàn)而(ér)成。天津益元利康科技有限(xiàn)公司在(zài)產品自主(zhǔ)研發的同時(shí),也與(yǔ)全(quán)球(qiú)多家生命科學(xué)試劑(jì)供應商建(jiàn)立(lì)良(liáng)好(hǎo)的合作關係(xì),基(jī)於“客戶第(dì)一(yī)"的核(hé)心理念,向全國10000+客戶(hù)提供200000+優質產(chǎn)品。
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